|
Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States Abstract The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures. |
|
Discovery of a novel
coronavirus associated with the recent pneumonia outbreak in humans and
its potential bat origin
Peng Zhou, Xing-Lou Yang, Xian-Guang Wang, Ben Hu, Lei Zhang, Wei
Zhang, Hao-Rui Si, Yan Zhu, Bei Li, Chao-Lin Huang, Hui-Dong Chen, Jing
Chen, Yun Luo, Hua Guo, Ren-Di Jiang, Mei-Qin Liu, Ying Chen, Xu-Rui
Shen, Xi Wang, Xiao-Shuang Zheng, Kai Zhao, Quan-Jiao Chen, Fei Deng,
Lin-Lin Liu, Bing Yan, Fa-Xian Zhan, Yan-Yi Wang, Gengfu Xiao, Zheng-Li
Shidoi: https://doi.org/10.1101/2020.01.22.914952 This article is a preprint and has not been certified by peer review [what does this mean?]. AbstractInfo/HistoryMetrics Preview PDF Abstract Since the SARS outbreak 18 years ago, a large number of severe acute respiratory syndrome related coronaviruses (SARSr-CoV) have been discovered in their natural reservoir host, bats. Previous studies indicated that some of those bat SARSr-CoVs have the potential to infect humans. Here we report the identification and characterization of a novel coronavirus (nCoV-2019) which caused an epidemic of acute respiratory syndrome in humans, in Wuhan, China. The epidemic, started from December 12th, 2019, has caused 198 laboratory confirmed infections with three fatal cases by January 20th, 2020. Full-length genome sequences were obtained from five patients at the early stage of the outbreak. They are almost identical to each other and share 79.5% sequence identify to SARS-CoV. Furthermore, it was found that nCoV-2019 is 96% identical at the whole genome level to a bat coronavirus. The pairwise protein sequence analysis of seven conserved non-structural proteins show that this virus belongs to the species of SARSr-CoV. The nCoV-2019 virus was then isolated from the bronchoalveolar lavage fluid of a critically ill patient, which can be neutralized by sera from several patients. Importantly, we have confirmed that this novel CoV uses the same cell entry receptor, ACE2, as SARS-CoV. |
dr Halat's global office Signal or WhatsApp +48 536 608 999 over WiFi or mobile networks
dr Z Halat, MD, medical epidemiology consultant, noxologist
For contact join gab or Telegram and search for @drhalat ; dr Halat Online - online medical consultation and advice Skype address: dr. halat. online ; For virtual online visit join Signal or WhatsApp and manually type in a new phone number: +48 536 608 999
text messages, photos, videos
over 3G/4G/LTE & Wi-Fi
Signal or WhatsApp +48 536 608 999 dr Halat global office
dr Halat Online - online medical consultation and advice
dr Halat Online - online medical consultation and advice Skype address: dr.halat.online
Skype
Get the new Skype for Windows on your computer.
It’s easy and it’s free to download.
| Novel
Coronavirus (2019-nCoV) contact options e-mail: medical(at)novel-coronavirus-2019-ncov.com
|
HEALTH RISK MANAGEMENT & COMMUNICATION
| VISIT
CHIROPTEROLOGY Bat research and study  chiropterology.com |
|
World Health Organization
declared that the outbreak of 2019-nCoV constitutes a Public Health
Emergency of International Concern (PHEIC)
see how they dealt with ebola crisis before and now ebola-virus-disease.com |